The universal primers and the shotgun DNA sequencing method.
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چکیده
منابع مشابه
Method for discovering novel DNA viruses in blood using viral particle selection and shotgun sequencing.
Rapid identification of viruses is needed to monitor the blood supply for emerging threats. Here we present a method that meets these criteria and allows for the shotgun sequencing of novel, uncultured DNA viruses directly from human blood. This method employs selection based on the physical properties of viruses combined with sequence-independent amplification and cloning. We show that both si...
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عنوان ژورنال:
- Methods in molecular biology
دوره 167 شماره
صفحات -
تاریخ انتشار 2001